Scioly.org

So I don't know if I'm just searching it wrong but I can't seem to find a place to buy Mini Toobers for modeling the structure. Could someone send me a link to where I can buy them?

Can someone please send us their files containing their work. I am really confused and would like to reference of someone. Thx x

Limke wrote:

greenMountainCoffee wrote:Hi,
This may be a stupid question, but on the JSMOL thing, it says the protein we should build is 84 residues long. Does that mean we should include residue #1 and include residue #84?

Sorry this was worded weirdly too

Yes, include residue 1 and 85*.
Also, just a clarification, the rules say to build residues 1-85.

Right but then... the protein would be 85 residues right?

RishiVanga wrote:So I don't know if I'm just searching it wrong but I can't seem to find a place to buy Mini Toobers for modeling the structure. Could someone send me a link to where I can buy them?

This https://www.3dmoleculardesigns.com/Scie ... g-Kits.htm is the site to buy a Mini Toober for the Pre-Build. You can also buy a practice kit that was the 2016 Regional On-site. This is the information page; click the blue "Buy Now" button to take you to a shopping page.

You know you don't have to buy the Mini Toobers. Less expensive options include 12 guage wire and this foam wire https://www.amazon.com/dp/B0026WGDYW/?c ... _lig_dp_it that Andy Hamm suggested to me. (It says size 12, but you just get one 32' roll.)

meierfra wrote:

RishiVanga wrote:So I don't know if I'm just searching it wrong but I can't seem to find a place to buy Mini Toobers for modeling the structure. Could someone send me a link to where I can buy them?

This https://www.3dmoleculardesigns.com/Scie ... g-Kits.htm is the site to buy a Mini Toober for the Pre-Build. You can also buy a practice kit that was the 2016 Regional On-site. This is the information page; click the blue "Buy Now" button to take you to a shopping page.

You know you don't have to buy the Mini Toobers. Less expensive options include 12 guage wire and this foam wire https://www.amazon.com/dp/B0026WGDYW/?c ... _lig_dp_it that Andy Hamm suggested to me. (It says size 12, but you just get one 32' roll.)

I would say that from an objective standpoint, the main advantage with buying the kits to build with would be that everything is cut to the exact length that the model needs to be, as well as the fact that it provides some supports and caps for the N and C termini, and the onsite practice kits also have model amino acid parts as well (although that's not necessarily the most efficient way to get amino acid residues, you can easily make them out of whatever material your heart desires within the scope of the rules).

It's more or less a convenience thing, if anything. I know plenty of models have been really good and high-scoring without using the kits as well. Totally up to you.

greenMountainCoffee wrote:

Limke wrote:

greenMountainCoffee wrote:Hi,
This may be a stupid question, but on the JSMOL thing, it says the protein we should build is 84 residues long. Does that mean we should include residue #1 and include residue #84?

Sorry this was worded weirdly too

Yes, include residue 1 and 85*.
Also, just a clarification, the rules say to build residues 1-85.

Right but then... the protein would be 85 residues right?

Yes.

I measured mine out to be 85 residues.

When has/will the On-Site model protein been/be determined? Is it supposed to be the CRISPR, since we're modelling anti-CRISPR for the pre-build? I understand that the specific section won't be given until competition.

The onsite build can be any protein they choose. (from what I understand)

daydreamer0023 wrote:When has/will the On-Site model protein been/be determined? Is it supposed to be the CRISPR, since we're modelling anti-CRISPR for the pre-build? I understand that the specific section won't be given until competition.

From the MSOE website, the onsite builds will be a specific section of the CRISPR Cas-9 protein from PDB File 5f9r.pdb.

Limke wrote:

greenMountainCoffee wrote:

Limke wrote:
Yes, include residue 1 and 85*.
Also, just a clarification, the rules say to build residues 1-85.

Right but then... the protein would be 85 residues right?

Yes.

I measured mine out to be 85 residues.

Okay, yes (sorry this is taking so long to marinate in my brain) but the toober is only 168 cm long in the kit. 168/2 = 84....? So we must not include the actual residue of either 1 or 85 correct?