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Re: Protein Modeling C

Posted: November 29th, 2018, 12:13 pm
by MacintoshJosh
Just to clarify, 5vw1.pdb has 3 chains, not 2.

Re: Protein Modeling C

Posted: November 29th, 2018, 8:11 pm
by ukhakimova3944
Hello!
Just to clarify, we are only building chain b? Looking at previous models the chain looks too small, so I am worried that we need to model the other chains. I read the previous posts, but being the anxious person that I am, I cannot help but ask.

Also, can the other chains be kept as functionally relevant parts if they are not part of the main build? I wanted to make them just in case and though I have not gotten to that, I may very well still model them and have the other members do so as practice.

Many thanks in advance.

Re: Protein Modeling C

Posted: November 30th, 2018, 2:46 pm
by juakk700
How do we figure out which terminal is which? I'm a bit confused.
Also, any general tips to start out?

Re: Protein Modeling C

Posted: November 30th, 2018, 3:24 pm
by MacintoshJosh
ukhakimova3944 wrote:Hello!
Just to clarify, we are only building chain b? Looking at previous models the chain looks too small, so I am worried that we need to model the other chains. I read the previous posts, but being the anxious person that I am, I cannot help but ask.

Also, can the other chains be kept as functionally relevant parts if they are not part of the main build? I wanted to make them just in case and though I have not gotten to that, I may very well still model them and have the other members do so as practice.

Many thanks in advance.


Yes, you are correct, we are building only chain B of the protein complex shown on Jmol (5vw1.pdb file). http://www.rcsb.org/structure/5VW1
Here is the link to the protein complex from the Protein Data Bank if you haven't already checked it out.
(Chain B is the anti-CRISPR protein.)

Now for your other question,

It is definitely not required to build parts from other chains but the rules sheet does say that 'Participants will use materials of their own choosing to add functionally relevant features to their model.'

Functionally relevant features could include parts from different chains but it's really up to you if you want to add those parts and if you find they are 'functionally relevant' and highlight the significance of structure to function of the protein.

Re: Protein Modeling C

Posted: November 30th, 2018, 3:47 pm
by MacintoshJosh
juakk700 wrote:How do we figure out which terminal is which? I'm a bit confused.
Also, any general tips to start out?

(Make sure you understand the event thoroughly, here's a good place to start: http://cbm.msoe.edu/scienceOlympiad/participantHome.php)

Research
I suggest first doing some research on proteins in general. Amino acids, levels of protein structure, sheets and helices, sidechains, hydrogen bonds, disulfide bonds, residues, peptide bonds, protein folding; these are all examples of some terms and concepts you should be familiar with before starting your model.

Once you understand everything about proteins, try researching on DNA. For example, genes, genomes, RNA, and specifically CRISPR and anti-CRISPR.

Jmol
Next, you need to be familiar with Jmol, which basically a program that allows you to view biological structures, in this case, the specified protein. http://cbm.msoe.edu/scienceOlympiad/designEnvironment/prebuild.html -- Link to Jmol and this year's protein. Practice the commands for Jmol with a reference sheet, located on the side of the Jmol model. Typing "restrict 1-85" then "restrict :B" will get you to the protein we are building this year (1-85 residues and B chain).

Building
CBM has some good videos on how to build your protein, I suggest checking them out.

Re: Protein Modeling C

Posted: December 2nd, 2018, 12:44 pm
by SOS_pls
Are we allowed to bring in a double-sided notecard or a single sided notecard to accompany our prebuild model?

Re: Protein Modeling C

Posted: December 2nd, 2018, 4:32 pm
by dnzsnr
Anyone know how to undo a command in the JSmol design environment? I've tried just typing "undo" or "undo <command>" but nothing seems to be working.

Re: Protein Modeling C

Posted: December 3rd, 2018, 4:21 pm
by eagerlearner102
Did any of you receive an orange strand for the protein modeling prebuild kit? The orange strand is longer than the blue strand. They said that it was because the blue strand wasn't the correct length. Did any of you get this?

Re: Protein Modeling C

Posted: December 3rd, 2018, 7:03 pm
by KSSOISLIT
Are y'all bringing in 3 sheets of notes at your invitationals? We've been using only 1 sheet, but someone pointed out to me recently that the rules, similar to the Forensics rules this year, say "each participant" before mentioning the "one sheet" comment. Would this mean a team of 3 can bring 3 sheets? Thanks.

Re: Protein Modeling C

Posted: December 3rd, 2018, 7:05 pm
by BanjoRodriguez
Hey all,

How much are the functional add ons weighted in the judging process? Also how many are recommended? The rules are quite ambiguous about it. :?:

I’ve been relatively successful with the event at the two invitationals I’ve been to, but I’m really trying to take my pre-build to the “next level” to remain competitive with some of the great teams in my region. Any suggestions? :D

Re: Protein Modeling C

Posted: December 3rd, 2018, 7:15 pm
by CookiePie1
KSSOISLIT wrote:Are y'all bringing in 3 sheets of notes at your invitationals? We've been using only 1 sheet, but someone pointed out to me recently that the rules, similar to the Forensics rules this year, say "each participant" before mentioning the "one sheet" comment. Would this mean a team of 3 can bring 3 sheets? Thanks.


Yes, that's correct. If you have a team of two, bring two sheets, etc.

BanjoRodriguez wrote:Hey all,

How much are the functional add ons weighted in the judging process? Also how many are recommended? The rules are quite ambiguous about it. :?:

I’ve been relatively successful with the event at the two invitationals I’ve been to, but I’m really trying to take my pre-build to the “next level” to remain competitive with some of the great teams in my region. Any suggestions? :D


As you go from regionals/invies to states and nats, there will be more and more weight placed on the added features. In general, you should add as many as you can that are functionally relavant to the protein. To further improve your model, you can spray paint your model to indicate the different secondary structures, and keep adding more add ons. You should also focus on the test to get as high a score as possible.

Re: Protein Modeling C

Posted: December 4th, 2018, 4:55 pm
by eagerlearner102
For those that had past experience, do any of you have suggestions on how to fold it? I watched the videos but I struggle with the 3D structure folding. It is so hard! :(

Re: Protein Modeling C

Posted: December 5th, 2018, 10:44 am
by JoeyC
Practice. Keep on doing it til you can't function. Rinse repeat, and folding comes natural

Re: Protein Modeling C

Posted: December 5th, 2018, 5:46 pm
by tm-scioli
Hello,
I am a bit confused as to add-ons. I have the main chain, end caps, and colored secondary structures, but I'm not sure what to add from here beyond putting in a hundred hydrogen bonds.
Thanks in advance!

Re: Protein Modeling C

Posted: December 5th, 2018, 6:05 pm
by CookiePie1
tm-scioli wrote:Hello,
I am a bit confused as to add-ons. I have the main chain, end caps, and colored secondary structures, but I'm not sure what to add from here beyond putting in a hundred hydrogen bonds.
Thanks in advance!


You should only add extra features that are functionally relevant to the protein. There's no use to adding so many hydrogen bonds because they aren't specific to the function of this particular protein.