Page 1 of 28

Protein Modeling C

Posted: June 30th, 2018, 11:56 am
by pikachu4919
Protein Modeling C: Students will use computer visualization and online resources to construct physical models of the CRISPR Cas9 protein that is being engineered to edit plant and animal cell genomes, and answer a series of questions about the chemistry of protein folding and the interaction of structure and function for model proteins.

Protein Modeling Wiki

http://cbm.msoe.edu/scienceOlympiad/

Official Protein Modeling Event Facebook Page (many photos of models from nationals are posted here)

Past Threads:
2011 (Pluripotent Stem Cells)
2012 (Apoptosis)
2015 (Human Genome Editing)
2016 (Dopamine and Serotonin Synthesis)

Past Question Marathons: 2015

Re: Protein Modeling C

Posted: September 4th, 2018, 10:00 pm
by luanne
Only first day after rules come out and I already have questions.. :roll:
so here they come:
1. In Jmol if I enter "restrict 1-85" I get around 4 distinct backbones. Is this correct?
2. How should I research and find which secondary structures are important to the function of the protein?
and lastly:
3. In jmol I would do the "select structures" and realize that one of the dna strands is also a beta sheet. So when I build my model, should I say that it is dna or a beta sheet?
(If you can't tell I am very lost)
Thanks :)

Re: Protein Modeling C

Posted: September 5th, 2018, 9:02 pm
by pikachu4919
Only first day after rules come out and I already have questions.. :roll:
so here they come:
1. In Jmol if I enter "restrict 1-85" I get around 4 distinct backbones. Is this correct?
2. How should I research and find which secondary structures are important to the function of the protein?
and lastly:
3. In jmol I would do the "select structures" and realize that one of the dna strands is also a beta sheet. So when I build my model, should I say that it is dna or a beta sheet?
(If you can't tell I am very lost)
Thanks :)
1. When I opened it and played around with it, I saw four beta pleated strands and one long alpha helix.
2. The cbm msoe website will upload lots of resources for that part, however, the name of the paper accompanies the PDB file info on RCSB, with that title you could probably search it on the web. If it doesn't grant you access, see if you can log in through your school's library system to get to it.
3. "select structures" is a pretty general command. Most of the time, you would do "select" and then your more specific kind of structure like "helix," "sheet," "dna," "rna," etc. then you can use Boolean operators to select multiple kinds of structures at once. I'm not entirely sure what you did that made it appear that way since the two are biochemically very different. But no, if it does not have amino acid structures, then it cannot be a beta sheet.

Re: Protein Modeling C

Posted: September 6th, 2018, 7:06 am
by Creationist127
Do we need to model all four of the strands? If so, how? If not, which one do we need to model? :?:

Re: Protein Modeling C

Posted: September 6th, 2018, 1:05 pm
by nervoussenior1
Only first day after rules come out and I already have questions.. :roll:
so here they come:
1. In Jmol if I enter "restrict 1-85" I get around 4 distinct backbones. Is this correct?
2. How should I research and find which secondary structures are important to the function of the protein?
and lastly:
3. In jmol I would do the "select structures" and realize that one of the dna strands is also a beta sheet. So when I build my model, should I say that it is dna or a beta sheet?
(If you can't tell I am very lost)
Thanks :)
1. When I opened it and played around with it, I saw four beta pleated strands and one long alpha helix.
2. The cbm msoe website will upload lots of resources for that part, however, the name of the paper accompanies the PDB file info on RCSB, with that title you could probably search it on the web. If it doesn't grant you access, see if you can log in through your school's library system to get to it.
3. "select structures" is a pretty general command. Most of the time, you would do "select" and then your more specific kind of structure like "helix," "sheet," "dna," "rna," etc. then you can use Boolean operators to select multiple kinds of structures at once. I'm not entirely sure what you did that made it appear that way since the two are biochemically very different. But no, if it does not have amino acid structures, then it cannot be a beta sheet.
Hm When i played around, i got 5 sheets. I think we have to restrict the chain to B (?). They still haven't updated the prebuild link that's on the event description.

Re: Protein Modeling C

Posted: September 6th, 2018, 3:58 pm
by luanne

Hm When i played around, i got 5 sheets. I think we have to restrict the chain to B (?). They still haven't updated the prebuild link that's on the event description.
in the rules it just said residue 1-85?

Re: Protein Modeling C

Posted: September 6th, 2018, 6:46 pm
by nervoussenior1

Hm When i played around, i got 5 sheets. I think we have to restrict the chain to B (?). They still haven't updated the prebuild link that's on the event description.
in the rules it just said residue 1-85?
Like on the rcsb website it says that chain B is the protein and chain A C D are nucleic acids/hybrids. And the description says to model residues 1-85 of the PROTEIN, so I just assumed that we restrict it to B. I may be wrong because it's my first time doing protein modeling.

Re: Protein Modeling C

Posted: September 6th, 2018, 6:47 pm
by nervoussenior1

Hm When i played around, i got 5 sheets. I think we have to restrict the chain to B (?). They still haven't updated the prebuild link that's on the event description.
in the rules it just said residue 1-85?
Like on the rcsb website it says that chain B is the protein and chain A C D are nucleic acids/hybrids. And the description says to model residues 1-85 of the PROTEIN, so I just assumed that we restrict it to B. I may be wrong because it's my first time doing protein modeling.

Re: Protein Modeling C

Posted: September 6th, 2018, 6:56 pm
by luanne
hmm, I guess we are going to have to wait until msoe uploads their work.. and its my first time too, but I was pretty sure if it was just chain 'b' it would say "Residue 1-85 of chain B"
I could be totally wrong tho :)

Re: Protein Modeling C

Posted: September 6th, 2018, 6:58 pm
by luanne
Also, rcsb hasn't updated their science Olympiad page? It is still on influenza from last year.