You absolutely need to include 1 and 85. Make sure the Toober is completely unwound. Sometimes if if's twisted a little, that will take up some length. Pull it all the way out, so you're sure it's at its maximum length. If it's still only 168cm, you should contact the MSOE people and ask for a replacement.Okay, yes (sorry this is taking so long to marinate in my brain) but the toober is only 168 cm long in the kit. 168/2 = 84....? So we must not include the actual residue of either 1 or 85 correct?Yes.Right but then... the protein would be 85 residues right?
I measured mine out to be 85 residues.
EDIT: Oops. They are sending you 168 cm and CBM gives a good explanation of why they only give you 168 cm on their Ask the CBM, question 52. https://cbm.msoe.edu/scienceOlympiad/participant2.php