# Potions and Poisons

 Potions and Poisons Chemistry & Lab Event Forum Threads 2019 2018 Tests 2019 2018 Test Exchange Archive There are no images available for this event Question Marathons 2018 2017 (Prelim) Division B Champion Solon Middle School This event was not held recently in Division C

Potions and Poisons is a Division B event for the 2018 and the 2019 season. It was previously a trial event in Pennsylvania, Washington, and the 2016 National Tournament. In Potions and Poisons, participants demonstrate their knowledge on specified substances', and chemical properties and effects with a focus on common toxins and poisons. Category C goggles are required in this event along with covering of skin from the neck down. An apron/lab coat is commonly used.

Example of C goggles, which completely cover the eyes and protect from fumes

WORK IN PROGRESS, PLEASE BE PATIENT!

## Topics covered

Event topics from the 2019 season rules include:

• Ionic/Covalent bonds with relation to conductivity
• Mixtures, solutions and compounds and separation of the components within them
• Chemical and physical properties and changes
• Balancing chemical equations
• Dilution's effect on toxicity
• Toxic spills and effects of their spread via water, wind, or gravity
• Identification of various poisonous organisms along with their toxic effects (listed on the rules)
• Effects/chemistry of common household toxins and chemicals

Lab tasks from the 2019 season rules include:

### Chromatography

Chromatography is one of the most common labs that you'll see at invitationals. It is a bit hard to explain, so bear with me please.

Chromatography is when you separate a mixture (i.e. markers, pens, organic juices) by passing it through a solution, which will most likely be water.

Chromatography diagram

There are many different types of chromatography such as gas or column chromatography, but in the lab task you'll only need to know about simple chromatography.

To start, on your chromatography paper, you'll need to draw a start line and the ink spot. The start line should be above or near the water line. Your start line should be ~2-3 cm from the bottom of the paper for accurate results. You'll then attach the paper to a stick or lean it against a stick in the beaker. The solution (water) should travel up the paper and separate the sample into different colors.

After roughly 10-20 minutes, depending on your paper, your chromatography should be done and you should take it out to dry. After that, you should mark the water line's end point and where the colors ended. You'll have to distinguish the amount of colors that are there and use the retention factor for each color. I recommend putting dots at the highest point of each color, and measuring it to find the distance.

The retention/retardation factor (RF) is as follows: math distance moved by solute/distance moved by solutemath or in simpler terms: math distance between color and start / distance water travelled math

If it doesn't work out the first few times for you, don't worry. It takes practice to perfect this technique, hence why Potions is labeled as a practice event.

### Mixtures of Reagents

Reagents are basically the starting materials used in chemical reactions. They're used to test if a reaction would occur. This type of lab is rare, but it can occur here and there.

These types of labs depend solely on the situation, and doesn't have a set procedure like the other labs. On the test, it should describe the exacts about the lab and what to do.

### Separation of Mixtures

This type of lab, like the previous example, depends solely on the situation. These mixtures are reversible, and you just need to determine which type of separation method to use.

The most common, and simplest methods are:

• Magnets - Separating magnetic metals from non-magnetic substances
• Filtering - Separating solid particles in solutions
• Decanting - Separating solid particles that have settled at the bottom of the heterogenous mixture by slowly pouring it and not disturbing the bottom particles
• Evaporating - For things like sugar/salt (anything soluble), you can evaporate the liquid and you'll be left with the substance
• Chromatography - Previously mentioned

### Dilution

Shown is a diagram of serial dilution, as you can see the concentration goes down with each dilution

Dilution is simply the addition of a solvent without adding any solute. This is shown in the equation:

$C_1 \times V_1 = C_2 \times V_2$

where

• C1 = the initial concentration of the solute
• V1 = the initial volume of the solution
• C2 = the final concentration of the solute
• V2 = the final volume of the solution

For example, if a solution has a 10% concentration of salt in one liter of water, adding another liter of water would halve the concentration of the salt, to 5%. This example can be shown mathematically using the above equation, where:

• C1 = 10%
• V1 = 1 L
• C2 = 5%
• V2 = 2 L

$10\% \times 1 L = 5\% \times 2L$

$0.1 \times 1 L = 0.05 \times 2L$

$0.1 L = 0.1 L$

So, for example when told to make a sample with a 1:1440 dilution factor, you'd take the solution and dilute it by 12, 12, and 10 respectively (12 * 12 * 10 = 1440) to get to that certain dilution.

### pH determination

pH is the potential of hydrogen, which basically is a number that decides how strong of a base or acid a solution is. The range of pH in water is 0-14, however with other substances it can go well below 0 or well above 14. In Potions, you'll probably be only working with the 1-14 scale. 0-6 pH is acidic, 7 is neutral, and 8-14 are bases.

To test the pH, you'd typically use litmus or pH paper. There are many ways of doing this, but the one thats most efficient is just dipping the paper in the water (about 10% of the paper should be submerged) and taking it out to dry on a spot plate. You would then match the color of the paper to the color on your litmus paper box/holder. If the paper just looks wet, it is neutral. Litmus/pH paper does not work with heavily dyed or dark colored liquids.

In pH, every number down (relative to water's scale) is 10 times stronger than the previous number. (I.e pH 3 is 10x stronger than pH 4; pH 4 is 100x stronger than pH 6; pH 6 is .01x stronger than pH 4)

### Conductivity testing

For conductivity, it's quite simple. All you need to do is have your conductivity meter.

You can either make your own or buy one. Cheaper conductivity meters come with LED lights which indicate the conductivity of the liquid, (I.e. dimmer may mean less conductive, brighter may mean more conductive) whereas electronic ones, which are more expensive, display a number. Not all conductivity meters are made the same, SO READ YOUR USER MANUAL.

To use it, you just submerge the end part of the conductivity meter into the solution. DO NOT GET ANY LIQUIDS ON THE ACTUAL METER. After use, turn it off and clean the part you submerged with a wet paper towel.

• Covalent bonds are not conductive, Ionic bonds are

## The Atom

• Electron
• Neutron
• Proton

### Shells, Subshells, and Orbitals

• Shells
• Subshells
• Orbitals
• Order of fillage

## The Periodic Table

For additional information about the periodic table, please see Chemistry Lab/Periodicity.

## Intramolecular bonding

A chemical bond is an attraction between two atoms that causes them to bond together, which can create molecules. The two types of bonding that the current version of the Potions and Poisons rules says to know are ionic and covalent bonding. Bonding occurs with electrons, in which the electrons are taken (ionic) or shared between atoms (covalent).

### Electronegativity

An electronegativity table

Electronegativity is a property of elements that is defined as the tendency of an atom to attract electrons. The difference between two atoms' electronegativities decides the type of bond they make. A chart known as an electronegativity chart can be used to find the electronegativities of different elements. Electronegativity increases as you go right and as you go up, therefore helium has the highest while francium has the lowest.

### Ionic Bonding

Ionic bonding is a type of bonding in which one atom takes an electron from another atom. This type of bonding occurs when the difference in electronegativity is high. A common example of this is the compound NaCl, or table salt. The metal Na (sodium) bonds with the halogen gas Cl (chlorine). When solid, ionic bonds are typically not conductive. However, when liquid, it is conductive.

Ionic bonds are typically formed with a crystal lattice structure and has a high melting temperature. They're also water soluble.

Shown is a venn diagram which demonstrates the difference between Ionic and Covalent bonds

To name a simple ionic compound, use the name of the regular metal, followed by the name of the nonmetal, with the latter using the ending "-ide". For example, NaCl would be written as sodium chloride.

### Covalent Bonding

Covalent bonding is a type of bonding in which atoms 'share' electrons. This occurs when there is a low difference in electronegativity. Two very common examples are the molecules H2 and O2. Because the bonds are formed between two atoms of the same element, the difference in their electronegativities must be zero. Covalent bonds are liquids and gases, however they are not conductive.

Covalent bonds are formed with a true molecule structure and has a low melting temperature. They are not water soluble (most of the time) and are odorous.

Covalent bonds can be nonpolar or polar. In polar covalent bonds, the atoms have different electronegativities, and therefore share electrons unequally. The more electronegative atom has a partial negative charge, while the less electronegative atom has a partial positive charge. In nonpolar covalent bonds, the atoms have similar electronegativities and therefore share the electrons equally. These are denoted with the δ symbol (Greek lowercase delta), using δ- for partial negative charge and δ+ for partial positive charge.

To summarize, polar covalent bonds share electrons unequally and therefore have poles. Nonpolar covalent bonds share electrons equally, and therefore do not have poles.

## Intermolecular Bonds

### Van Der Waals

The weakest of the three,

### Hydrogen Bonding

The strongest of the three,

## Chemical Equations

Chemical reactions are written out as chemical equations. A chemical equation has two parts: reactants and products. The atoms in the reactants are rearranged to form the products.

Chemical equations are written left-to-right with the products following the reactants, and an arrow sign (which is read as "yields") pointing from the reactants to the products. Each individual reactant/molecule is represented with a plus sign (+). The following equation is an example of a chemical equation, with the reactants on the left and the product on the right.

$2H_2 + O_2 \rightarrow 2H_2O$

If there are multiple instances of a molecule, the number of molecules is written as a coefficient. For example, the product in the above equation is water, or H₂O. There are two water molecules present, which is written as 2H₂O.

### Balancing Chemical Equations

In a chemical reaction, the quantity of each element cannot change (If there are n atoms of element A in the reactants, there must be n atoms in the product). A chemical equation must have equal quantities of each element on either side of the arrow. As mentioned above, adding coefficients to molecules can show that there are those many molecules present. However, if an equation is given without coefficients, chances are that there is an inequality on either side. Consider the example given above; however this time it is without coefficients:

$H_2 + O_2 \rightarrow H_2O$

If you count the number of each element on either side of the equation, you will get the following:

• Hydrogen on left: 2
• Oxygen on left: 2
• Hydrogen on right: 2
• Oxygen on right: 1

This cannot be a balanced equation, because the number of atoms is unequal. To fix this issue, it is necessary to balance the chemical equation.

To balance a chemical equation, add coefficients to make the number of atoms of each element equal. For example, take again the previous equation:

$H_2 + O_2 \rightarrow H_2O$

Notice that there is only one type of molecule as the product, meaning that it is the only molecule that a coefficient can be added to. A basic way to find the proper coefficient is to find a ratio between the two elements on one side and apply that to the other side. In this example, there are two times as many hydrogens as oxygens. In addition, all coefficients must be whole numbers. Therefore, the lowest coefficients would be a two in front of the hydrogen gas (on the left) and a two in front of the water. This gives us a balanced equation of:

$2H_2 + O_2 \rightarrow 2H_2O$

An easy way to go about this is to guess and check the distinct elements first. For example:

mathC3H8 + O2 -> CO2 + H2Omath

you should start with C as it's only present in two compounds that don't involve O, which is involved in 3. If putting 3 at CO2 doesn't work, double it.

When the answer to the blank is 1, you should leave it blank. Only fill in >1. When the equation is already balanced, just write "balanced"

## Poisonous Plants and Animals

The poisonous plants and animals listed in the 2019 rules are:

• Poison ivy (Toxicodendron radicans)
• Wild Carrot (Daucus carota)
• Autumn Skullcap (Galerina marginata)
• Henbane (Hyoscyamus niger)
• Oak (Quercus sp)
• Timber Rattlesnake (Crotalus horridus)
• Eastern Coral Snake (Micrurus Fulvius)
• Cotton Mouth Snake (Agkistrodon piscivorus)
• Black Widow Spider (Latrodectus mactans)
• Brown Recluse Spider (Locosceles recluse)

What is included is a general description of the organism, the poisonous effects, and a brief description of the poison itself. It is encouraged that you research further, however.

### Poison Ivy (Toxicodendron radicans)

Poison ivy grows most regions of the US, typically in woods, fields, and along roadsides, especially where vegetation is disturbed. It can be identified by its three thin, pointy, and shiny leaves. The leaf color depends on the season. In the spring, the leaves are reddish; in the summer, green; in the fall, orange to bronze.

An example of the "three leaf rule" for poison ivy.

Upon contact with the oil from poison ivy, an allergic reaction happens. Touching the plant itself is not the only way to contact the oil; touching gardening equipment or pets that have contacted the ivy can also spread the oil. Symptoms of a reaction include itching, redness, swelling, and blisters. It is important to note that the blisters are NOT contagious.

The poison, Urushiol, is an oily mixture of organic compounds with allergenic properties. It can also be found in mango trees, lacquer trees, and other Anacardiaceae plants. It gets its name from the Japanese word for the lacquer tree, urushi. The urushiol allows the tree to form a hard lacquer which is used in Asian lacquerware. It's a pale yellow liquid which has a boiling point of 200C. It can easily be removed with soap and water, provided that the skin hasn't absorbed it yet.

### Wild Carrot (Daucus carota)

Wild carrot, also known as bird's nest, bishop's lace, and Queen Anne's lace, grows in Europe, Southeast Asia, and is naturalized in North America and Australia. Domesticated carrots, like the ones in the store, are cultivars of a subspecies. They're biennial plants that grow between 30-60 cm and are hairy with solid stems. Their leaves are tripinnate and separate into thin segments. The flowers are small, and clustered in flat, dense umbels. They made be pink, and have a red/purple flower.

An example of the leaves of the wild carrot

Wild carrots are edible in their early stages, but become stiff and woody. Their flowers are edible throughout their entire life, however, and are battered and fried commonly. The leaves may cause phytophotodermatitis, a cutaneous phototoxic inflammatory reaction resulting from contact with a light sensitive agent. It can also be used as a contraceptive and dye. It has a bitter taste that usually results from surrounding crops, like apples.

Wild carrot contains acetone, asarone, choline, ethanol, fomic acic, HCN, isobutyric acid, limonen, malic acid, maltose, oxalic acid, palmitic acid, pyrrolidine, and quinic acid. The amounts of the toxins are rather small, but it causes intoxication to large farm mammals such as horses or cattle. Contact with the carrot can cause skin irritation when exposed to the sun. Carotoxin is also found in wild carrot, making it toxic to mice and Daphnia magna. It can also be infected by some diseases, making consumption deadly.

### Past Plants and Animals

Plants and animals that have been included in past years are listed below.

 2016-2017 (Trial Rules) A common misconception is to believe that the Portuguese man o' war (a hydrozoan) is a jellyfish. Wolfsbane (Aconitum sp.) Wolfsbane is also known as Aconite or Monkshood. It is an herbaceous, perennial plant that grows well in mountain meadows. The flowers are large and usually blue, purple, white, yellow, or pink. Although poisonous, some species are used for ornamental purposes. Signs of poisoning include nausea, vomiting, diarrhea, tingling, and numbness. If a fatal dose is taken, death occurs within 2-6 hours. Poisoning can also occur just by touching the leaves without gloves, because the toxin aconitine is absorbed easily through the skin. Jack in the Pulpit (Arum maculatum) Jack in the Pulpit, also known as Indian turnip, is found in deciduous woods and floodplains. The flower is green and maroon striped, and the seed is bright red. The leaves and fruit contain calcium oxalate (CaC2O4) which is irritating to the skin, so gloves should be worn when handling this plant. Jack in the Pulpit is also irritating when ingested raw, and contact with the roots can cause skin blisters. However, the roots can be safely eaten when cooked. Lily of the Valley (Convallaria majalis) Lily of the Valley is an herbaceous perennial plant that spreads through underground rhizomes. It flowers in late Spring, and the flowers consist of 6 white tepals (outer parts of the flower). The flowers have a sweet scent. The fruit is a small reddish berry. All parts of the plant, including flowers and berries, are poisonous. Any amount ingested can cause abdominal pain, vomiting, and blurred vision. Many cardiac glycosides, medications that increase heart output while decreasing contraction rate, are derived from this plant. Poison Sumac (Toxicodendron vernix) Poison Sumac is a small shrub/tree that grows in wet clay soils such as swamps and peat bogs. It has reddish tinted leaves and greenish flowers. All parts of it contain the resin urushiol, which causes skin and mucous membrane irritation. Symptoms on the skin can include swelling, inflammation, and oozing. Poison Sumac can be especially dangerous when burned, because it can lead to pulmonary edema. The urushiol contained in this plant is similar to that contained in Poison Ivy, but the Poison Sumac urushiol tends to be more toxic. Poison Dart Frog (Dendrobates sp.) Poison Dart Frogs are small and poisonous frogs. They inhabit northern South America, as well as some of Central America. Poison dart frog poison is neurotoxic and cardiotoxic, and can harm organisms that come in contact with the skin of the organism. This type of poisoning can cause convulsions and heart arrhythmia, which eventually leads to heart failure. Portuguese Man o' War (Physalia physalis) Portuguese Man o' War are usually thought of as a type of jellyfish, but they are siphonophores. Whereas jellyfish are single organisms, the man o' war is made up of many unique creatures. Not to be confused with Physalia utriculus, Physalia physalis is found in the Atlantic. The part of the creature that remains afloat while the rest of it is submerged is the pneumatophore. Portuguese man o' war can't move on their own and move with the wind, current, or tide, making them planktonic. The barbed nematocysts of this creature contain neurotoxic venom which usually cause red welts in individuals that come in contact with them. In extreme cases, man o' war stings can cause death. These nematocysts can be used by other organisms for beneficial purposes, however. For example, blanket octopuses many be seen carrying broken man o' war tentacles because they are immune to the venom.

## Common Household Toxins

The 2019 rules of Potions and Poisons mention the following toxic household chemicals:

• Ammonia
• Hydrogen peroxide
• Rubbing alcohol
• Bleach
• Epsom salts
• Vinegar
• Nutritional supplements containing calcium and iron

### Ammonia

The compound ammonia itself is a colorless gas with the formula NH3. However, it is most commonly seen in households as a cleaner, where the gas is dissolved into water. It is most dangerous when mixed with bleach, which causes the release of toxic fumes, which can cause serious respiratory damage, in addition to potential chemical burns, headaches, nausea, or vomiting.

### Hydrogen peroxide

A typical "brown bottle" of hydrogen peroxide

Hydrogen peroxide is a colorless liquid. Its compound name is H2O2. It is commonly used as a disinfectant, either for surfaces or for wounds. Because the concentration of most hydrogen peroxide used in households (usually in brown bottles) is low, at about 3%, ingestion of small amounts of hydrogen peroxide (diluted) does not usually cause any significant damage, apart from potential stomach irritation. However, ingesting a large quantity can cause more serious stomach irritation and may even cause chemical burns.

Furthermore, ingestion of a higher concentration of hydrogen peroxide can cause much more serious symptoms, and death in some cases.

### Rubbing Alcohol

Rubbing alcohol (Isopropyl alcohol) is an alcohol with the formula C3H8O. It is most often used as a disinfectant. When ingested, it is metabolized into acetone. This can cause dizziness, headaches, vomiting, or even coma.

### Bleach

Bleach is a solution of the chemical compound sodium hypochlorite (NaClO) in water. It is a strong base, with a pH of 12.6. It is most often used as a household cleaner. As mentioned above, mixing bleach with ammonia releases dangerous fumes. Exposure to bleach on its own can cause irritation in the eyes, mouth, skin, and lungs, and can cause burns.

### Epsom Salts

Epsom salts (Magnesium sulfate) are salts with the equation MgSO4. They have many uses, including uses as bath salts, as laxatives, face cleansers, cleaners, and as fertilizer. However, ingesting high levels of these salts can cause magnesium overdose, which can lead to slowed heartbeat, lowered blood pressure, nausea, vomiting, and coma or death in serious cases.

### Vinegar

Vinegar is an extremely common household chemical. It is an acidic liquid, a mixture of acetic acid (CH3COOH) and water. It is used in cooking, cleaning, and medicine. However, concentrations of acetic acid higher than 10% can cause skin damage/corrosion.

### Nutritional Supplements Containing Calcium and Iron

An excess of calcium, known as hypercalcemia, may result from overuse of calcium supplements. Symptoms of hypercalcemia include nausea, thirst, lethargy, and muscle weakness; in severe cases, cardiac arrhythmias and palpitations may occur.

Iron supplements are often taken for anemia, but iron poisoning can occur and be potentially fatal, especially in children under 5 years old. The GI tract and stomach become irritated and internal cell reactions may be interrupted due to an excess of iron. Vomiting and nausea are some of the earliest symptoms; if untreated, the liver may develop severe scars and fail. It can be treated through bowel irrigation or chelation therapy.

## 2016 National Tournament Trial Events

 Division B: Hovercraft · Geocaching · Potions and Poisons | Division C: Code Busters · Mystery Design · Remote Sensing